SAM Protocols

See below for detailed methods and protocols.

CRISPR/Cas9 Synergistic Activation Mediator (SAM) pooled human library

Here we describe how to amplify the human SAM library to have sufficient quantity to produce lentivirus, while maintaining full library representation. Protocol details can be downloaded as a pdf.

A spreadsheet with NGS primers for library readout can be found here: zip of xlsx.

CRISPR/Cas9 Synergistic Activation Mediator (SAM) for individual gene targeting

A description of how to clone individual sgRNAs for SAM activation for both the lenti-sgRNA(MS2)_EF1A-zeo cloning backbone (addgene #61427) and the sgRNA(MS2) cloning backbone (addgene #61424) pdf.